rabbit anti ho1  (Proteintech)

Journal: Journal of Thoracic Disease

Article Title: Protective effects of estradiol on neuroinflammation in chronic obstructive pulmonary disease related depression

doi: 10.21037/jtd-2025-247

Figure Lengend Snippet: Oxidative stress and inflammation level increased in the hippocampus in COPD-related depression in rat model. (A) The antioxidant protein expression images of rat hippocampus. (B-D) Qualitative analysis of Nrf2, HO1 and NQO1. (E) The Bcl2/Bax expression images of rat hippocampus. (F) Qualitative analysis of Bcl2/Bax. (G,H) qualitative analysis of p-NFκB and TNF-α. (I) The p-NFκB and TNF-α protein expression images of rat hippocampus. Data are presented as mean ± SEM, n=3. All statistical tests were conducted using two-way ANOVA. *, P<0.05, **, P<0.01, ***, P<0.001. ANOVA, analysis of variance; COPD, chronic obstructive pulmonary disease; CS, cigarette smoke; CTL, control; HO1, heme-oxygenase-1; NQO1, quinone oxidoreductase 1; Nrf2, nuclear factor erythroid 2-related factor 2; OVX, ovariectomy; p-NFκB, phosphorylated nuclear factor kappa B; SEM, standard error of the mean; TNF-α, tumor necrosis factor α.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20) and then incubated overnight at 4 °C with primary antibodies: β-actin (1:20,000, Proteintech, #66009-1-Ig), nuclear factor erythroid 2-related factor-2 (Nrf2) (1:1,000, Proteintech, #80593-1), quinone oxidoreductase 1 (NQO1) (1:1,000, ABclonal, #A19586), heme-oxygenase-1 (HO1) (1:1,000, ABclonal, #A19062), phosphorylated nuclear factor kappa B (p-NFκB) (1:1,000, CST, #3033S), NFκB(1:1000, CST, #8242S), extracellular signal-regulated kinase 1/2 (ERK1/2) (1:1,000, Cell Signaling Technology, #4695), Phospho-ERK1/2 (1:2,000, Cell Signaling Technology, #4370), c-Jun-N-terminal kinase (JNK) (1:1,000, Cell Signaling Technology, #9252), Phospho-JNK (1:1,000, Cell Signaling Technology, #4668), Phospho-p38 (1:1,000, Cell Signaling Technology, #4511), p38 (1:1,000, Cell Signaling Technology, #9212), tumor necrosis factor α (TNF-α) (1:1,000, ABclonal, #A0277), inducible nitric oxide synthase (iNOS) (1:1,000, ImmunoWay, #YT3169), CD163 (1:1,000, ABclonal, #A8383), and CD86 (1:1,000, ABclonal, #A1199).

Techniques: Expressing, Control

Journal: Journal of Thoracic Disease

Article Title: Protective effects of estradiol on neuroinflammation in chronic obstructive pulmonary disease related depression

doi: 10.21037/jtd-2025-247

Figure Lengend Snippet: E2 promotes BV2 cell MAPKs activation and M1 polarization . (A) WB images of anti-oxidative stress protein level in BV2 cells stimulated with LPS and/or E2. (B-D) qualitative analysis of Nrf2, HO-1 and NQO1. (E) WB images of BV2 cells polarization stimulated with LPS and/or E2. (F,G) Qualitative analysis of CD163 and iNOS. (H) WB images of MAPK signaling pathway protein level in BV2 cells stimulated with LPS and/or E2. (I-K) Qualitative analysis of p-JNK, p-ERK1/2, p-p38. Data are presented as mean ± SEM, n=3. All statistical tests were conducted using two-way ANOVA. *, P<0.05, **, P<0.01, ***, P<0.001. ANOVA, analysis of variance; CTL, control; E2, estradiol; HO1, heme-oxygenase-1; iNOS, inducible nitric oxide synthase; LPS, lipopolysaccharide; NQO1, quinone oxidoreductase 1; Nrf2, nuclear factor erythroid 2-related factor 2; p-ERK1/2, phosphorylated extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated c-Jun-N-terminal kinase; SEM, standard error of the mean; WB, Western blotting.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20) and then incubated overnight at 4 °C with primary antibodies: β-actin (1:20,000, Proteintech, #66009-1-Ig), nuclear factor erythroid 2-related factor-2 (Nrf2) (1:1,000, Proteintech, #80593-1), quinone oxidoreductase 1 (NQO1) (1:1,000, ABclonal, #A19586), heme-oxygenase-1 (HO1) (1:1,000, ABclonal, #A19062), phosphorylated nuclear factor kappa B (p-NFκB) (1:1,000, CST, #3033S), NFκB(1:1000, CST, #8242S), extracellular signal-regulated kinase 1/2 (ERK1/2) (1:1,000, Cell Signaling Technology, #4695), Phospho-ERK1/2 (1:2,000, Cell Signaling Technology, #4370), c-Jun-N-terminal kinase (JNK) (1:1,000, Cell Signaling Technology, #9252), Phospho-JNK (1:1,000, Cell Signaling Technology, #4668), Phospho-p38 (1:1,000, Cell Signaling Technology, #4511), p38 (1:1,000, Cell Signaling Technology, #9212), tumor necrosis factor α (TNF-α) (1:1,000, ABclonal, #A0277), inducible nitric oxide synthase (iNOS) (1:1,000, ImmunoWay, #YT3169), CD163 (1:1,000, ABclonal, #A8383), and CD86 (1:1,000, ABclonal, #A1199).

Techniques: Activation Assay, Control, Western Blot